Table 3. PASMC fluxes in 21% and 0.5% oxygen.
| Type | Pathway | ID | Reaction | 21%* | 0.5%† | Ratio | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Flux | LB | UB | Flux | LB | UB | |||||
| NET | Transport | GLUT | GLC.x → GLC | 4.28E+02 | 4.28E+02 | 4.28E+02 | 3.65E+02 | 3.65E+02 | 3.65E+02 | 0.85 |
| PYRR | PYR.x → PYR.c | 1.04E+02 | 1.02E+02 | 1.09E+02 | 4.53E+01 | 4.31E+01 | 4.57E+01 | 0.44 | ||
| MCT | LAC ↔ LAC.x | 8.01E+02 | 8.01E+02 | 8.04E+02 | 6.49E+02 | 6.49E+02 | 6.49E+02 | 0.81 | ||
| ALAR | ALA → ALA.x | 1.43E+01 | 1.43E+01 | 1.46E+01 | 7.83E+00 | 7.83E+00 | 8.24E+00 | 0.55 | ||
| GLNR | GLN.x → GLN | 7.73E+01 | 7.53E+01 | 7.73E+01 | 1.77E+02 | 1.77E+02 | 1.77E+02 | 2.29 | ||
| GLUR | GLU ↔ GLU.x | 2.53E+01 | 2.52E+01 | 2.54E+01 | 1.19E+01 | 1.19E+01 | 1.22E+01 | 0.47 | ||
| ASPR | ASP → ASP.x | 7.01E+00 | 6.99E+00 | 7.02E+00 | 6.92E+00 | 6.84E+00 | 7.00E+00 | |||
| SERR | SER.x → SER | 2.54E+00 | 2.48E+00 | 2.55E+00 | 2.57E+00 | 2.55E+00 | 2.57E+00 | 1.01 | ||
| CYSR | CYX.x → CYS +CYS | 6.39E+00 | 6.34E+00 | 6.45E+00 | 3.75E+00 | 3.75E+00 | 3.75E+00 | 0.59 | ||
| GLYR | GLY → GLY.x | 3.66E-01 | 3.03E-01 | 4.19E-01 | 4.06E-01 | 3.86E-01 | 4.25E-01 | |||
| Glycolysis | HK | GLC → G6P | 4.28E+02 | 4.28E+02 | 4.28E+02 | 3.65E+02 | 3.65E+02 | 3.65E+02 | 0.85 | |
| PGI | G6P ↔ F6P | 4.06E+02 | 4.06E+02 | 4.07E+02 | 3.62E+02 | 3.62E+02 | 3.63E+02 | 0.89 | ||
| PFK | F6P → FBP | 4.17E+02 | 4.17E+02 | 4.18E+02 | 3.61E+02 | 3.60E+02 | 3.61E+02 | 0.87 | ||
| ALDO | FBP ↔ DHAP +GAP | 4.17E+02 | 4.17E+02 | 4.18E+02 | 3.61E+02 | 3.60E+02 | 3.61E+02 | 0.87 | ||
| TPI | DHAP ↔ GAP | 4.16E+02 | 4.16E+02 | 4.16E+02 | 3.60E+02 | 3.60E+02 | 3.60E+02 | 0.87 | ||
| GAPDH | GAP ↔ 3 PG | 8.39E+02 | 8.39E+02 | 8.41E+02 | 7.21E+02 | 7.21E+02 | 7.21E+02 | 0.86 | ||
| ENO | 3 PG → PEP | 8.36E+02 | 8.35E+02 | 8.53E+02 | 7.20E+02 | 7.20E+02 | 7.20E+02 | 0.86 | ||
| PK | PEP → PYR.c | 9.31E+02 | 9.30E+02 | 9.31E+02 | 9.24E+02 | 9.24E+02 | 9.24E+02 | 0.99 | ||
| LDH | PYR.c ↔ LAC | 8.01E+02 | 8.01E+02 | 8.04E+02 | 6.49E+02 | 6.49E+02 | 6.49E+02 | 0.81 | ||
| GPT1 | PYR.c ↔ ALA | 1.64E+02 | 1.62E+02 | 1.92E+02 | –1.36E+01 | –1.39E+01 | –1.35E+01 | –0.08 | ||
| GPT2 | PYR.m ↔ ALA | –1.43E+02 | –1.43E+02 | –1.42E+02 | 2.62E+01 | 2.51E+01 | 2.65E+01 | –0.18 | ||
| Pentose phosphate pathway | G6PD | G6P → P5P+CO2 | 1.89E+01 | 1.57E+01 | 1.93E+01 | 1.16E-07 | 0.00E+00 | 1.10E-03 | 0 | |
| TK1 | P5P+P5 P ↔ S7P+GAP | 5.46E+00 | 4.44E+00 | 5.96E+00 | –6.15E-01 | –6.15E-01 | –5.77E-01 | –0.11 | ||
| TA | S7P+GAP ↔ F6P+E4 P | 5.46E+00 | 4.44E+00 | 5.96E+00 | –6.15E-01 | –6.15E-01 | –5.77E-01 | –0.11 | ||
| TK2 | P5P+E4 P ↔ F6P+GAP | 5.46E+00 | 4.44E+00 | 5.96E+00 | –6.15E-01 | –6.15E-01 | –5.77E-01 | –0.11 | ||
| Anaplerosis | PYRT | PYR.c → PYR.m | 7.60E+01 | 7.59E+01 | 7.66E+01 | 3.36E+02 | 3.36E+02 | 3.36E+02 | 4.42 | |
| PC | PYR.m+CO2 → OAC | 6.30E+01 | 6.29E+01 | 6.59E+01 | 2.37E+02 | 2.36E+02 | 2.37E+02 | 3.76 | ||
| PEPCK | OAC → PEP +CO2 | 9.51E+01 | 9.51E+01 | 9.53E+01 | 2.03E+02 | 2.03E+02 | 2.04E+02 | 2.14 | ||
| ME2 | MAL → PYR.m +CO2 | 1.20E-03 | 0.00E+00 | 5.20E-03 | 1.82E+02 | 1.81E+02 | 1.82E+02 | 151517.08 | ||
| ME1 | MAL → PYR.c +CO2 | 3.29E-05 | 0.00E+00 | 1.15E+00 | 5.91E-05 | 0.00E+00 | 8.06E-02 | |||
| FAO | FAO → AcCoA.m | 1.00E-07 | 0.00E+00 | 1.32E-02 | 1.15E-04 | 0.00E+00 | 1.56E-01 | |||
| GLDH | GLU ↔ AKG | 4.43E+01 | 4.42E+01 | 4.45E+01 | 1.59E+02 | 1.59E+02 | 1.59E+02 | 3.6 | ||
| GLS | GLN ↔ GLU | 7.38E+01 | 7.36E+01 | 7.38E+01 | 1.74E+02 | 1.74E+02 | 1.74E+02 | 2.36 | ||
| Tricarboxylic acid cycle | PDH | PYR.m → AcCoA.m +CO2 | 1.56E+02 | 1.48E+02 | 1.66E+02 | 2.55E+02 | 2.55E+02 | 2.55E+02 | 1.63 | |
| CS | AcCoA.m+OAC → CIT | 1.56E+02 | 1.56E+02 | 1.58E+02 | 2.55E+02 | 2.55E+02 | 2.55E+02 | 1.63 | ||
| IDH | CIT ↔ AKG +CO2 | 2.11E+01 | 2.10E+01 | 2.11E+01 | 2.16E+01 | 2.16E+01 | 2.16E+01 | 1.03 | ||
| OGDH | AKG → SUC +CO2 | 6.54E+01 | 6.51E+01 | 6.59E+01 | 1.81E+02 | 1.80E+02 | 1.81E+02 | 2.77 | ||
| SDH | SUC ↔ FUM | 6.54E+01 | 6.51E+01 | 6.59E+01 | 1.81E+02 | 1.80E+02 | 1.81E+02 | 2.77 | ||
| FH | FUM ↔ MAL | 6.54E+01 | 6.51E+01 | 6.59E+01 | 1.81E+02 | 1.80E+02 | 1.81E+02 | 2.77 | ||
| MDH | MAL ↔ OAC | 2.01E+02 | 2.01E+02 | 2.01E+02 | 2.32E+02 | 2.32E+02 | 2.33E+02 | 1.16 | ||
| GOT | OAC ↔ ASP | 1.22E+01 | 1.17E+01 | 1.24E+01 | 1.07E+01 | 1.06E+01 | 1.07E+01 | 0.87 | ||
| Amino acid metabolism | PST | 3 PG → SER | 2.69E+00 | 2.57E+00 | 2.80E+00 | 7.12E-01 | 7.01E-01 | 7.21E-01 | 0.26 | |
| SHT | SER ↔ GLY +MEETHF | 5.19E+00 | 5.15E+00 | 5.20E+00 | 3.82E+00 | 3.81E+00 | 3.86E+00 | 0.74 | ||
| CYST | SER ↔ CYS | –1.12E+01 | –1.17E+01 | –1.11E+01 | –6.35E+00 | –6.35E+00 | –6.35E+00 | 0.57 | ||
| SD | SER → PYR.c | 6.39E+00 | 6.23E+00 | 6.44E+00 | 2.33E+00 | 2.33E+00 | 2.33E+00 | 0.36 | ||
| GLYS | CO2 +MEETHF → GLY | 2.39E+00 | 2.36E+00 | 2.42E+00 | 1.80E+00 | 1.79E+00 | 1.81E+00 | 0.75 | ||
| Biomass | BIOMASS | 978*AcCoA.c+237.8* ALA +187*ASP +92.3* CO2 +57.46*CYS +45.97*DHAP +114.5*G6P+127.6* GLN +153*GLU +260.8*GLY +101.1*MEETHF +92.3*P5P+174.8* SER → biomass |
2.77E-02 | 2.70E-02 | 2.79E-02 | 2.00E-02 | 2.00E-02 | 2.00E-02 | 0.72 | |
| ACL | CIT → AcCoA.c +MAL | 1.35E+02 | 1.34E+02 | 1.38E+02 | 2.33E+02 | 2.33E+02 | 2.33E+02 | 1.72 | ||
| LIPS | AcCoA.c → lipid | 1.08E+02 | 9.99E+01 | 1.08E+02 | 2.14E+02 | 2.14E+02 | 2.14E+02 | 1.98 | ||
| Mixing | cPYR | 0*PYR.c → PYR.ms | 5.77E-01 | 5.64E-01 | 5.92E-01 | 1.00E+00 | 9.96E-01 | 1.00E+00 | 1.73 | |
| mPYR | 0*PYR.m → PYR.ms | 4.23E-01 | 4.08E-01 | 4.36E-01 | 1.00E-07 | 0.00E+00 | 4.40E-03 | 0 | ||
| sPYR | PYR.ms → PYR.fix | 1.00E+00 | 1.00E+00 | 1.00E+00 | 1.00E+00 | 1.00E+00 | 1.00E+00 | |||
| EXCH | Transport | MCT | LAC ↔ LAC.x | 1.00E-07 | 0.00E+00 | 1.36E+02 | 1.64E+03 | 1.63E+03 | 1.65E+03 | 16400000000 |
| GLUR | GLU ↔ GLU.x | 1.00E-07 | 0.00E+00 | 2.27E-02 | 5.69E-05 | 0.00E+00 | 1.71E-02 | |||
| Glycolysis | PGI | G6P ↔ F6P | 4.88E+06 | 4.88E+06 | Inf | 9.92E+06 | 9.85E+04 | Inf | ||
| ALDO | FBP ↔ DHAP +GAP | 2.89E+02 | 2.80E+02 | 2.89E+02 | 2.57E+02 | 2.56E+02 | 2.57E+02 | 0.89 | ||
| TPI | DHAP ↔ GAP | 9.86E+06 | -Inf | Inf | 1.65E+03 | 1.63E+03 | 1.68E+03 | |||
| GAPDH | GAP ↔ 3 PG | 1.12E+03 | 0.00E+00 | 5.88E+05 | 1.00E-07 | 0.00E+00 | 2.27E-01 | |||
| LDH | PYR.c ↔ LAC | 1.47E+03 | 1.39E+03 | 1.47E+03 | 4.49E+02 | 4.49E+02 | 4.49E+02 | 0.31 | ||
| GPT1 | PYR.c ↔ ALA | 2.74E+02 | 2.73E+02 | 2.77E+02 | 1.00E-07 | 0.00E+00 | 4.28E-02 | 0 | ||
| GPT2 | PYR.m ↔ ALA | 1.38E+02 | 1.38E+02 | 1.49E+02 | 9.64E+01 | 0.00E+00 | 1.01E+02 | 0.7 | ||
| Pentose phosphate pathway | TK1 | P5P+P5 P ↔ S7P+GAP | 7.99E+02 | 7.97E+02 | 8.08E+02 | 3.54E+01 | 3.54E+01 | 3.55E+01 | 0.04 | |
| TA | S7P+GAP ↔ F6P+E4 P | 1.53E-01 | 0.00E+00 | 5.82E-01 | 2.55E+00 | 2.54E+00 | 2.57E+00 | 16.67 | ||
| TK2 | P5P+E4 P ↔ F6P+GAP | 3.33E+00 | 2.62E+00 | 3.35E+00 | 1.29E+01 | 1.29E+01 | 1.29E+01 | 3.88 | ||
| Anaplerosis | GLDH | GLU ↔ AKG | 5.36E+02 | 5.34E+02 | 8.37E+02 | 1.23E+03 | 1.23E+03 | 1.23E+03 | 2.29 | |
| GLS | GLN ↔ GLU | 3.20E-01 | 0.00E+00 | 2.74E+00 | 1.12E+00 | 1.07E+00 | 1.74E+00 | |||
| Tricarboxylic acid cycle | IDH | CIT ↔ AKG +CO2 | 1.04E+01 | 1.02E+01 | 1.04E+01 | 6.30E+01 | 6.30E+01 | 6.31E+01 | 6.09 | |
| SDH | SUC ↔ FUM | 2.78E-01 | 0.00E+00 | Inf | 3.34E+06 | 3.34E+06 | 3.34E+06 | |||
| FH | FUM ↔ MAL | 1.03E-04 | 0.00E+00 | 1.58E+01 | 2.18E+02 | 2.18E+02 | 2.18E+02 | 2114238.83 | ||
| MDH | MAL ↔ OAC | 1.01E+03 | 8.27E+02 | 1.01E+03 | 3.67E+03 | 3.67E+03 | 3.69E+03 | 3.63 | ||
| GOT | OAC ↔ ASP | 2.27E+02 | 2.27E+02 | 2.47E+02 | 1.54E+01 | 1.54E+01 | 1.55E+01 | 0.07 | ||
| Amino acid metabolism | SHT | SER ↔ GLY +MEETHF | 3.55E+00 | 3.52E+00 | 3.59E+00 | 1.60E-01 | 1.36E-01 | 1.70E-01 | 0.05 | |
| CYST | SER ↔ CYS | 1.04E+03 | 1.03E+03 | 1.04E+03 | 2.00E-03 | 0.00E+00 | 2.00E-03 | 0 | ||
*
SSR 575.6 [499.1–630.6] (95% CI, 563 DOF).
†
SSR 521.3 [482.2–611.6] (95% CI, 545 DOF).