Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 19;20(7):1037–1047. doi: 10.1038/s41592-023-01903-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 4 — a, Comparison of the original (black and blue lettering) and multiplex (black and red lettering) GAM protocols. WGA: Whole Genome Amplification. b, Effect of various staining protocols on downstream DNA extraction. Top row: propidium iodide (n=21 each), SYBR® Gold (n=16 stained; 14 unstained) and Eosin (n=16 stained; 19 unstained); bottom row: crystal violet (n=16 stained; 15 unstained) and cresyl violet (n=16 stained; unstained the same as for crystal violet). Red lines indicate the median percentage of mapped reads per GAM-3NP sample. c, Top panel: cryosection thickness can be identified from the section’s color. Bottom panels: cresyl violet staining improves the visualization of NPs during laser microdissection. Scale bar 10μm. d, Top: unstained cryosections from mES cells. Individual NPs are outlined by dashed white lines. White arrows indicate typical background (air bubbles) in the microdissection membrane. Bottom: Cresyl violet stains both cytoplasm and nucleoplasm in mES cell cryosections as visualized by brightfield microscopy, and therefore does not distinguish cellular profiles that intersect the nucleus. Scale bar 10μm. e, Example mES cell cryosection visualized by confocal fluorescence microscopy. DNA stained with DAPI is shown in blue and RNA stained with SYTO® RNASelect dye is shown in green. White arrows indicate cellular profiles that do not intersect nuclei (n=647 profiles intersect the nucleus of 857 profiles analyzed; Supplementary Table 10). Scale bar 10μm. f, Binomial distribution showing the expected number of profiles per GAM sample that intersect nuclei across a collection of samples each with four cellular profiles.