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. 2023 Jul 10;13:11105. doi: 10.1038/s41598-023-37702-z

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© The Author(s) 2023, , corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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1 µM of SR144528 reduces LPS/IFN-γ-induced microglial inflammatory cytokine secretion independent of the CB2 receptor. (A) Experimental setup. Primary microglia of WT and CB2^-/- mice were pre-treated with 1 µM SR144528 for 15 min, followed by LPS/IFN-γ stimulation for 16 h. (B–D) Levels of cytokines/ chemokines were quantified in supernatants using ELISA. (B) TNF-α, (C) IL-6, and (D) CCL2. N = 4–5 samples/genotype/stimulation (representative data from two independent microglia preparations). Data are presented as mean ± SD. Two-way ANOVA followed by Tukey's multiple comparisons. #### p < 0.0001, ## p < 0.01, # p < 0.05 indicate significance to the untreated control group. Significant differences between LPS/IFN-γ vs. LPS/IFN-γ pre-treated with SR144528 are indicated with *** p < 0.001, ** p < 0.01, * p < 0.05.