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. 2023 Jul 10;13:11105. doi: 10.1038/s41598-023-37702-z

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© The Author(s) 2023, , corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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1 µM of SR144528 does not affect the percentage of live cells in LPS/IFN-γ stimulated microglia. (A) Experimental setup. Primary microglia were pre-treated with 1 µM SR144528 for 15 min, followed by LPS/IFN-γ stimulation for 16 h. DRAQ7™ was used to stain dead cells (B) Gating strategy used for the stained microglia. (C) Quantification of the percentage of live microglia cells (DRAQ7 negative). N = 6–9 samples/stimulation (from at least two independent microglia preparations). Data are presented as mean ± SD. One-way ANOVA followed by Tukey's multiple comparisons. ## p < 0.01, # p < 0.01 indicate significance to the untreated control group.