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. 2023 Jul 10;13:11105. doi: 10.1038/s41598-023-37702-z

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© The Author(s) 2023, , corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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SR144528 does not alter 3D microglial morphology in LPS/IFN-γ stimulated OHSCs. (A) Experimental setup. OHSCs were pre-treated with SR144528 at the indicated concentrations for 15 min, followed by LPS/IFN-γ stimulation for 16 h. After stimulation, OHSCs were stained for microglial markers and imaged using confocal microscopy. Microglia morphology was analyzed based on Iba1 staining using MotiQ. (B) Representative images of cropped and 3D reconstructed microglia, scale bar = 30 µm. (C–F) Quantification of morphological parameters of reconstructed microglia. (C) Branch number, (D) Tree length, (E) Ramification index, (F) Volume. N ≥ 40 microglial cells/stimulation (representative data from two independent OHSCs preparations). Data are presented as mean ± SD. One-way ANOVA followed by Tukey's multiple comparisons was used for normally distributed data, while Kruskal–Wallis test followed by Dunn's multiple comparisons tests was used for data that were not normally distributed. #### p < 0.0001 indicates significance to the untreated control group. Significant differences between LPS/IFN-γ vs. LPS/IFN-γ pre-treated with SR144528 are indicated with **** p < 0.0001, *** p < 0.001, ** p < 0.01.