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. 2023 Jul 10;8:33. doi: 10.1038/s41536-023-00311-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Dcx, mCherry, and BrdU triple immunostaining with DAPI counterstain in Lv13-transduced wild type mouse dentate gyrus. a1. mCherry channel of a. a2. Dcx and DAPI channel of a. a3. BrdU channel of a. b Dcx, mCherry, and BrdU triple immunostaining with DAPI counterstain in Lv16-transduced wild type mouse dentate gyrus. b1. mCherry channel of b. b2. Dcx and DAPI channel of b. b3. BrdU channel of b. c Dcx, mCherry, and BrdU triple immunostaining with DAPI counterstain in Lv13-transduced APP/PS1dE9 mouse dentate gyrus. c1. mCherry channel of c. c2. Dcx and DAPI channel of c. c3. BrdU channel of c. d Dcx, mCherry, BrdU triple immunostaining with DAPI counterstain in Lv1613-transduced APP/PS1dE9 mouse dentate gyrus. d1. mCherry channel of d. d2. Dcx and DAPI channel of d. d3. BrdU channel of d. e Normalized values of Dcx-positive, BrdU-Dcx double positive and BrdU-Dcx-mCherry triple positive cells in wild type mouse dentate gyrus transduced with Lv13 (control) or Lv16 (NGFR). f Normalized values of Dcx-positive, BrdU-Dcx double positive and BrdU-Dcx-mCherry triple positive cells in APP/PS1dE9 mouse dentate gyrus transduced with Lv13 (control) or Lv16 (NGFR). Scale bars equal 50 μm. Error bars represent standard error of the means, with each point representing one section. Y-axes represent numbers per mm². 3 mice were used.