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. 2023 Jul 10;13:11180. doi: 10.1038/s41598-023-38084-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Histopathological and ultrastructural analysis of low-grade glioma. (A) HE staining shows increased cellularity with predominant gliofibrillar (gf) component. Blood vessels (bv) show non-hyperplasic endothelial cells. (B) GFAP and (C) Ki67 immunostaings. (D) Immunofluorescence; acetylated β tubulin (red) is a marker of axoneme while pericentrin (green) is present in basal body. Colocalisation of both markers show clearly the presence of primary cilia (arrows). (E) Electron micrograph of a nuclear envelope-limited chromatin sheets (ELCS) isolating heterochromatin (N: nucleus, n: nucleoli, rer: rugous endoplasmic reticulum). (F) Electron micrograph of abundant parallel gliofilaments (gf) along an astrocytic prolongation. (G) Electron micrograph of abnormal disposition of gliofilaments acquired a characteristic concentric shape. (H) Electron micrograph of multivesicular bodies (mvb) present in glioma astrocytes (Go: Golgi apparatus, m: mitochondria).