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. 2023 Jun 27;14:1160783. doi: 10.3389/fmicb.2023.1160783

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Copyright © 2023 Sun, Zhang, Liu, Liu, Zhang, Fan, Zhang, Li and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immumohistochemical analysis stain for assessing 5-HT-positive cells expression in IBS-D rats. (A) IHC of 5-HT in the colon (scar bar: 100 μm), (B) MOD of 5-HT in the colon, (C) IHC of 5-HT in the hippocampus (scar bar: 1000 μm), (D) MOD of 5-HT in the hippocampus. The data were represented as mean ± SEM. **p < 0.01 versus NC group; ^##p < 0.01 versus MC group; ^bp < 0.01 versus CKF + PFK group. NC, control group; MC, model control group; CKF, Chang-Kang-Fang group; PFK, Bifico group; PFK-TM, Bifico target-colon capsule group; CKF-D + PFK, Chang-Kang-Fang combined with Bifico group; CKF-D + PFK-TM, Chang-Kang-Fang combined with Bifico target-colon capsule group; 5-HT, serotonin; MOD, mean optical density; CA3, cornu amonis 3; DG, dentate gyrus.