Table 3.
Organisms, main plasmid host and mobility, associated with the different variants of Tn5393 (Tn5393a to Tn5393l and Tn5393n).
| Organism | Tn5393 variant | ARGs carried by the transposon | Other MGEs present in the transposon | Plasmid; mobility; other ARGs carried by the plasmid | GenBank acc. Number | Reference(s) |
|---|---|---|---|---|---|---|
| E. amylovora | Tn5393 | strA-strB | IS1133 | pEa34; conjugative; none | M96392, M95402 | Chiou (1991), Chiou and Jones (1993), and McManus et al. (2002) |
| Tn5393 | strA-strB | IS1133 | pEa29; non-conjugative; none | NA | McManus et al. (2002) | |
| Tn5393a | strA-strB | None | pEU30; conjugative; none | NA | Foster et al. (2004) and Förster et al. (2015) | |
| P. syringae | Tn5393a | strA-strB | None | pPSR1; conjugative; none | AY342395 | Foster et al. (2004) and Sundin et al. (2004) |
| X. campestris pv. vesicatoria | Tn5393b | strA-strB | IS6100 | pBV5-4a; ND; none | U20588 | Sundin and Bender (1995) and Sundin (2002) |
| A. salmonicida | Tn5393c | strA-strB | None | pRAS2; conjugative; sul2, tetA(31) and tetR(31) | AF262622 | L’Abée-Lund and Sørum (2000) |
| A. faecalis | Tn5393d | strA-strB, aphA6b, blaPER-1 | IS1012L and Tn4176 including IS1012R, IS1387, IS1066, ISPa12, ISPa13, ISPpu17 | pFL424; non-conjugative; none | AJ627643 | Mantengoli and Rossolini (2005) |
| S. enterica sv. Typhimurium | Tn5393a | strA-strB | None | pSRC27-I (I1 type plasmid); conjugative; various (e.g., blaCMY-2, aadA, aac3, sul1, tetB, tetAR, blaTEM-1, aphA) | CP058811 | Harmer (2021) |
| Tn5393e | strA-strB, aphA1b | Tn6023 including IS26 | pSRC125; ND; sul1, dfrA5, tetA(B) | GU562437 | Cain and Hall (2011) | |
| Tn5393k | strA-strB, aph(3′)-Ib | IS26, IS1 | pSW39; conjugative; aadA2, aph(3′)-Ia, aac(6′)-Ib-cr, blaDHA-1, blaOXA-1, arr-3, tetA, floR, catB4, qnrB4, aac(6′)-Ib-cr, sul1, sfrA12, mphA | NA | Yao et al. (2021) | |
| Tn5393l | strA-strB, tetD(B), tetC(B), tetA(B), tetR(B) | IS1133, Tn10 including IS10 | R64; conjugative; none | AP005147 | Zhang et al. (2019) | |
| Unidentified soil bacteria | Tn5393f | strA-strB | IS26 | pHH1107; conjugative; gene homologous to tetX, sul2 | FJ012881 | Heuer et al. (2009), and Cain and Hall (2011) |
| Unidentified soil bacteria | Tn5393g | strA-strB, aacC3 | ISKpn12 | pHHV35; conjugative; aacC2, sul2 | FJ012882 | Heuer et al. (2009) and Cain and Hall (2011) |
| S. enterica sv. Kentucky | Tn5393h | strA-strB, tetA(B) and tetR | IS1133 and Tn10 including IS10 | pCVM29188_146; conjugative; none | CP001122 | Fricke et al. (2009) and Cain and Hall (2011) |
| Yersinia ruckeri | Tn5393i | strA-strB, tetA(B) and tetR | IS1133 including Tn10, IS10 and IS903 | pYR1; probably conjugative (carries genes essential for conjugative transfer); sul2, dhfrl | CP000602 | Welch et al. (2007) and Cain and Hall (2011) |
| K. pneumoniae | Tn5393j | strA-strB, aphA6 | ISPa14 | pJEG011; conjugative; blaOXA-48, blaCTX-M-14 | KC354801 | Espedido et al. (2013) |
| Aeromonas sp. | Tn5393k’ | strA-strB, aph(6)-Id, aph(3′)-VIb, aph(3″)-Ib, blaPER-1 | IS1012L and Tn4176 including IS1012R, IS1387, IS1066, ISPa12, ISPa13 | ND | NA | Adamczuk and Dziewit (2017) |
| A. caviae | Tn5393n | strA-strB | ISAeca7 | Chromosomic | CP084031 | Luo et al. (2022) |
E. amylovora, P. syringae and X. campestris pv. vesicatoria are PPB, while Aeromonas sp., A. faecalis, S. enterica, Yersinia ruckeri and K. pneumoniae are human, animal, pathogenic or environmental bacteria. ND, not determined; NA, not available. Note that Tn5393k was described twice but the two versions were not identical and are distinguished here as Tn5393k and Tn5393k’. The genetic organization of these Tn5393 variants is illustrated in Figure 2.