Fig. 7.

SD2267 attenuated acetaminophen (APAP)-induced liver injury in mice

(A) Experimental schedule of the APAP-induced liver injury mouse model. After 16 h of fasting, mice were injected with APAP (250 mg/kg, IP) and 2 h later treated with SD2267 (1 or 3 mg/kg, IP) for another 6 h. (B) Serum AST and ALT levels are presented as means ± SEMs. (C) Protein expression levels of liver tissues were determined by western blotting, and densitometry results of each protein levels are presented as means ± SEMs (n = 3 per group). (D) GSH to GSSG ratio was detected from liver tissues, and presented as means ± SEMs (n = 3 per group). (E) Representative images of hematoxylin and eosin (HE) stained liver tissues (left) with measured areas of necrosis (presented as means ± SEMs) (right). Necrotic areas are marked with dashed lines in HE images. (F) Representative images of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) stained liver tissues (left) and the number of TUNEL positive cells per high power field (HPF; x400) (presented as means ± SEMs) (right). cv; central vein. Scale bar = 100 μm *p < 0.05, **p < 0.01, ***p < 0.001.