Reagent/Resource	Reference or Source	Identifier or Catalog Number
Experimental Models
Escherichia coli BL21(DE3) gold	Agilent Technology	Cat. 230132
Escherichia coli OmniMAX	ThermoFisher	Cat. C854003
Escherichia coli SS320	Lucigen	Cat. 60512‐1
M13KO5 helper phage	ThermoFisher	Cat. 18311019
Flp‐In T‐RExHeLa cells	Stephen Taylor
Recombinant DNA
pETM33	EMBL
pETM41	EMBL
Phagemid p8	Sidhu lab (PMID: 25879139)
HURP cDNA	CPR clone resource
YFP pcDNA/FRT/TO	Nilsson lab – cloning YFP with HindIII in pcDNA5/FRT/TO
Antibodies
HRP‐conjugated anti‐M13 bacteriophage monoclonal mouse antibody	Sino Biological Inc	Cat. 11973‐MM05T‐H/RRID: AB_2857928
anti‐CHC polyclonal rabbit antibody	Bethyl Laboratories	Cat. A304‐743A‐M/RRID: AB_2782136
anti‐HURP polyclonal rabbit antibody	Bethyl Laboratories	Cat. A300‐852‐M/RRID: AB_2779502
anti‐GFP (mouse/rabbit) antibody	rabbit‐ anti GFP, produced in‐house or mouse anti‐GFP from Roche	Cat. 11814460001/RRID: AB_390913 for Roche
IRDye® 800CW Goat anti‐Mouse IgG Secondary Antibody	LI‐COR	Cat. 926‐32210/RRID: AB_621842
IRDye® 680RD Goat anti‐Rabbit IgG Secondary Antibody	LI‐COR	Cat. 926‐68071/RRID:vAB_10956166
Oligonucleotides and sequence‐based reagents
RNA oligos/Ambion™ Silencer™ Select Pre‐Designed siRNA	Thermo Fisher	s18913
Oligonucleotides PM_HD2	CustomArrray
HURP_fwd_in_YFP	GATCGGATCCATGTCTTCATCACATTTTGCC
HURP_rev_in_YFP_wt	GATCGCGGCCGCTCAAAATTCTCCTGGTTGTAGAGG
HURP_rev_in_YFP_delta835‐846	GATCGCGGCCGCTCAGTTACCACCAAAAGAAATGTGTC
HURP_fwd_S839A	GGTAACCTGATTACTTTTGCACCTCTACAACCAGGAG
HURP_rev_S839A	CTCCTGGTTGTAGAGGTGCAAAAGTAATCAGGTTACC
SiRes_HURP_fwd_483	GATAACGAGAGTGACGTGAGGGCGATCCGACCTGGTCC
SiRes_HURP_rev_483	GGACCAGGTCGGATCGCCCTCACGTCACTCTCGTTATC
SiRes_HURP_fwd_484	ATGCCGGTCCTCAAAACACAAAGAGTGAACATGTGAAG
Sires_HURP_rev_484	CTTCACATGTTCACTCTTTGTGTTTTGAGGACCGGCAT
Chemicals, enzymes and other reagents
Zeocin	Invitrogen	Cat. R25001
Blasticidin	Invitrogen	Cat. R21001
Doxycyclin	Clontech Labs	631311 – 5g
Nocodazole	Sigma	Cat. M1404
Lipofectamine™ RNAiMAX Transfection Reagent	Invitrogen	Cat. 13778‐150
Hygromycin B	Invitrogen	Cat. 10687010
0.25 % Trypsin/EDTA	ThermoFisher	Cat. 25200‐056
DMEM GlutaMax 4.5 g/l D‐Glucose, Pyruvate	Thermo Fisher	Cat. 31966047
OPTIMEM medium	Invitrogen	Cat. 51985026
GFP‐Trap Agarose	YChromotek	Cat. GTA‐20
PhosSTOP Easypack	Roche	Cat. 04906837001
Thymidine	Sigma	Cat. T1895
IGEPAL CA‐630	Sigma	Cat. I8896
Protein Assay Dye Reagent Concentrate	Bio‐Rad	Cat. 5000006
NuPAGE LDS Sample buffer (4×)	Novex	Cat. NP0008
MOPS SDS Running Buffer (20×)	Invitrogen	Cat. NP0001‐02
cOmplete™ EDTA‐free Protease Inhibitor Cocktail	Roche	Cat. 4693132001
T4 polynucleotide kinase	Thermo Scientific	Cat. EK0031
T7 DNA polymerase	Thermo Scientific	Cat. EP0081
T4 DNA ligase	Thermo Scientific	Cat. EL0014
50 bp marker	Thermo Scientific	Cat. 10416014
Mag‐bind Total Pure NGS	Omega Bio‐tek	Cat. M1378‐01
QIAquick Gel extraction Kit	Qiagen	Cat. 28706X4
Quant‐iT PicoGreen dsDNA Assay Kit	Molecular probes by Life technologies	Cat. P7589
TMB substrate	Seracare KPL	Cat. 5120‐0047
QIAquick Nucleotide Removal Kit	Qiagen	Cat. 28306
GSH Sepharose 4 Fast Flow Media	Cytiva	Cat. 17513201
Ni Sepharose excel	Cytiva	Cat. 17371201
FastDigest SmaI	ThermoFisher	Cat. FD0663
Affinity chromatography columns, HiTrap™ Benzamidine FF	Cytiva	Cat. 17514302
Thrombin	Cytiva	Cat. 27‐0846‐01
Thermo Scientific™ Lysozyme	ThermoFisher	Cat. 89833
GelRed	Biotium	Cat. 41003‐T
Thermo Scientific™ Phusion High‐Fidelity PCR Master Mix with HF Buffer	ThermoFisher	Cat. F631L
QIAquick PCR Purification Kit	Qiagen	Cat. 28104
jetOPTIMUS® DNA transfection reagant and buffer	Polyplus	Cat. 101000006
96‐well Flat‐bottom Immunosorp MaxiSorp plates	Nunc, Roskilde, Denmark	Cat. 439454
384‐well Flat‐bottom Immunosorp MaxiSorp plates	Nunc, Roskilde, Denmark	Cat. 464718
96‐well half area black Flat‐bottom Nonbinding surface plates	Corning, USA	Cat. 3993
100 × 20 cm Greiner Cellstar Cell Culture Dish	Greiner Bio‐One	Cat. 664160
145 × 20 cm Greiner Cellstar Cell Culture Dish	Greiner Bio‐One	Cat. 639160
μ‐slide 8 well	Ibidi	Cat. 80826
Software
GraphPad Prism version 9.3.1 for MacOS	GraphPad Software, San Diego, CA, USA, www.graphpad.com
ImageJ	https://imagej.nih.gov/ij/
PyMOL Version 2.1.1	New York, NY, USA Schrodinger LLC
DeltaVision Softworx Software	DeltaVision
Python version 3	Van Rossum and Drake (2009)
Pandas	McKinney (2010)
Matplotlib	Hunter (2007)
Seaborn	Waskom et al (2021)
Inkscape	Inkscape Project (2020). Inkscape. Retrieved from https://inkscape.org
Other
iD5	Molecular Devices
iTC200	Malvern
PCR machine	Biometra TGradient
Illumina MiSeq v3 run, 1 × 150 bp read setup, 20% PhiX	NGS‐NGI SciLifeLab facility
Nanodrop ND‐1000	Thermo Fisher
DeltaVision Microscope	DeltaVision