Reagent/Resource	Reference or source	Catalog number
Experimental models
E. coli MG1655	Lee et al (2018)
DICON 053, 055, and 088	Kanamori et al (2017)
Recombinant DNA
p15A‐pTet‐sfGFP‐linker‐Tdimer‐kanR	This study
miniTn7‐HJD1B	Xia et al, 2018
Chemicals, enzymes, and other reagents
Sodium phosphate dibasic	Sigma	S3264
Potassium phosphate monobasic	Sigma	P5655
Sodium chloride	Sigma	S3014
Ammonium chloride	Sigma	A9434
Glucose	Acros organics	388190010
Thiamine hydrochloride	Sigma	T1270
Magnesium sulfate	Sigma	M2643
Calcium Chloride	OmniPur	10035‐04‐8
Casamino Acid	BD	223120
Kanamycin sulfate	Sigma	60615
Carbenicillin, Disodium Salt	Genesee Scientific	25‐532
Amoxicillin	Sigma	A8523
Cefotaxime sodium salt	Sigma	C7039
Potassium clavulanate	Sigma	33454
LB Broth (Miller) Mix	Genesee Scientific	11‐120
Bacto Yeast Extract	BD	212750
Bacto Tryptone	BD	211705
UltraPure™ Low Melting Point Agarose	Invitrogen	16520050
Software
MATLAB R2021a
Python 3.8
Fiji	https://fiji.sc/
Other
Tecan Infinite 200 Pro	Tecan
Keyence BZ‐X710, BZ‐X800	Keyence
ThermalSeal RTS	Excel Scientific
Gene frame	Thermofisher scientific
Press‐To‐Seal Silicon Isolator	Grace Bio‐Labs