Table 1.

Primer Sequences for Methylation-Specific PCR Chain Reaction used for BRCA1 Gene Promoter

Gene	Primer Name	Sense Primer	Antisense Primer	Annealing Temp (oC)	Size (bp)
BRCA1	Unmethylated	GGTTAATTTAGAGTTTTGAGAGATG	TCAACAAACTCACACCACACAATCA	61	182 bp
	Methylated	GGTTAATTTAGAGTTTCGAGAGACG	TCAACGAACTCACGCCGCGCAATCG	65	182 bp