Table 4.
Overview of CE methods used for analysis of SCFAs.
| Matrix | Pretreatment | Separation capillary | BGE | Detection | LOD | LOQ | Ref. |
|---|---|---|---|---|---|---|---|
| Feces | Aqueous extraction | Silica capillary coated with polyacrylamide, 37 cm total length, 50 μm ID | 234 mM phosphate buffer (pH 6.10) with 12% (v/v) methanol | UV (200 nm) | – | 16.1 -49.6 µM | (181) |
| Faeces (mice) | Acidification, aqueous extraction | Fused silica capillary, 55 cm effective length, 60 cm total length, 75 um ID | 160 mM tris(hydroxymethyl)aminomethane + 10 mM benzoic acid (pH 8.5) | Indirect UV (228 nm) | 0.03 – 0.19 mM | 0.09 – 0.43 mM | (182) |
| Faeces (human, canine) | Aqueous extraction | Fused-silica capillary, 71.5 cm effective length, 80 cm total length, 75 um ID | 10 mM β-alanine + 10 mM benzoic acid (pH 5.7) + 4 mM α-CD + 0.005% polybrene | Indirect UV (350 nm) | — | 8 – 10 μM | (183) |
| Faeces (rat) | Aqueous extraction | Fused-silica capillary, 56 cm effective length, 64.5 cm total length, 75 um ID | 5 mM α-CD, 8 mM 5’-AMP, 7.5% (v/v) MeOH, 100 mM boric acid (pH 6.5) |
Indirect UV (320 nm) | — | — | (184) |
| Feces | Liquid-liquid extraction, Lyophilization | Fused-silica capillary, 49 cm effective length, 58 cm total length, 50 um ID | 0.5 mM tetradecyl trimethyl ammonium bromide (TTAB) + 10 mM 1,3(6,7)-naphthalenetrisulfonate (NTS) + 5.0 mM 1,5-naphthalenedisulfonic acid (NDS) + 5.0 mM sodium chromate + 10 mM of diethylamine (DEA) (pH 11.5) | Indirect UV (280 nm) | 1.2 – 3.6 μM | 4 – 12 μM | (185) |