Fig. 2.
Fluorescence analysis of the cellular uptake of courmarine-6-labeled C-LPs and Rg3-LPs in MCF-7/T cells. (A) Confocal fluorescence images of MCF-7/T cells in the presence of C-LPs or Rg3-LPs. (B) FACS analysis of cellular uptake of the liposomes. (C) Cellular uptake efficiency of C-LPs and Rg3-LPs. (D) In vitro binding of Rg3-LPs to MCF-7/T cells. Representative fluorescence images of Rg3-LPs and GLUT-1 localization in MCF-7/T cells. (E) Cellular uptake of Rg3-LPs in the presence of GLUT inhibitors relative to the control as determined by flow cytometry. Scale bar, 50 μm, Data are mean ± SD, n = 3. *p < 0.05, **p < 0.01, ***p < 0.001.