Table 1. . Characterization of ebselen-unloaded and ebselen-loaded core-shell alginate nanocapsules.
| Oil phase composition | Ebselen-unloaded core-shell alginate nanocapsules | Ebselen-loaded core-shell alginate nanocapsules | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Labrafac lipophile WL1349 (g) | Span 80 (g) | Transcutol HP (g) | Code | Diameter (nm) | PdI | ζ potential (mV) | Code | Diameter (nm) | PdI | ζ potential (mV) | EE (%) |
| 0.417 | 0.05 | 0.083 | NCS 1 | 211 ± 2 | 0.22 ± 0.01 | -20.8 ± 0.1 | Eb-NCS 1 | 204 ± 1 | 0.15 ± 0.01 | -15.4 ± 0.1 | 45.1 ± 2.1 |
| 0.400 | 0.05 | 0.100 | NCS 2 | 198 ± 3 | 0.20 ± 0.02 | -20.0 ± 0.1 | Eb-NCS 2 | 194 ± 3 | 0.17 ± 0.01 | -15.9 ± 0.2 | 41.3 ± 2.7 |
| 0.375 | 0.05 | 0.125 | NCS 3 | 172 ± 2 | 0.16 ± 0.01 | -21.1 ± 0.3 | Eb-NCS 3 | 184 ± 1 | 0.12 ± 0.02 | -15.9 ± 0.1 | 38.4 ± 0.9 |
| 0.333 | 0.05 | 0.167 | NCS 4 | 166 ± 1 | 0.16 ± 0.01 | -20.0 ± 0.2 | Eb-NCS 4 | 179 ± 1 | 0.11 ± 0.01 | -15.5 ± 0.5 | 29.3 ± 1.5 |
| 0.250 | 0.05 | 0.250 | NCS 5 | 160 ± 1 | 0.15 ± 0.01 | -20.4 ± 0.3 | Eb-NCS 5 | 172 ± 1 | 0.11 ± 0.01 | -17.1 ± 0.9 | 26.9 ± 1.2 |
To obtain nanocapsules, 0.55 g of oil phase was dispersed in 1.955 g of water phase, followed by the sonication for 2 min. Further, still under probe sonication, the entire volume (0.5 ml) of aqueous CaCl 2 solution (0.7 mg/ml) was instantaneously injected and the ultrasonication process was further continued for another 3 min. Mean ± standard deviation, n = 3.
Eb-NC: Ebselen-loaded nanocapsule; EE: Encapsulation efficiency; PdI: Polydispersity index.