Skip to main content
. 2023 Jan 15;56(7):e13400. doi: 10.1111/cpr.13400

FIGURE 4.

FIGURE 4

Non‐SMC condensin I complex subunit H (NCAPH) affects PD‐L1 expression by regulating the stability of β‐catenin protein. (A and B) The co‐IP assay was performed to detect the endogenous protein interaction between NCAPH and β‐catenin in 786‐O and Caki‐1 cells. (C) The treatment of MG132 blocked the change of β‐catenin level mediated by NCAPH overexpression or depletion. (D) Ubiquitination assay of β‐catenin in Caki‐1 cells co‐transfected with Myc‐Ub and Flag‐β‐catenin and treated with MG132. (E) PD‐L1 mRNA expression in clear cell renal cell carcinoma (ccRCC) cells was measured after NCAPH depletion or β‐catenin overexpression. ***p < 0.001 versus sh‐NC + vector; ### p < 0.001 versus sh‐NCAPH‐1. (F) The PD‐L1 protein level in ccRCC cells was measured after NCAPH depletion or β‐catenin overexpression. (G) Membrane PD‐L1 level in ccRCC cells was measured after NCAPH depletion or β‐catenin overexpression by flow cytometry. Each experiment was repeated at least three times.