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. 2023 Jan 15;56(7):e13400. doi: 10.1111/cpr.13400

FIGURE 5.

FIGURE 5

Non‐SMC condensin I complex subunit H (NCAPH) is related to aerobic glycolysis in clear cell renal cell carcinoma (ccRCC). (A and B) Glucose consumption and lactate production of ccRCC cells was measured. (C) The effect of NCAPH‐β‐catenin axis on the extracellular acidification rate (ECAR) in 786‐O and Caki‐1 cells. (D–G) The effect of NCAPH‐β‐catenin axis on the mRNA level of glycolysis‐related genes (HK2, PKM2, LDHA and GLUT1) in ccRCC cells. ***p < 0.001 vssh‐NC + vector; ### p < 0.001 versus sh‐NCAPH‐1. (H) Cell viability of ccRCC cells was measured by CCK‐8 assay after NCAPH overexpression or 2‐DG treatment. (I) EDU‐positive cells were counted in ccRCC cells after NCAPH overexpression or 2‐DG treatment. (J) The number of clones was counted in ccRCC cells with NCAPH overexpression or 2‐DG treatment. ***p < 0.001 versus vector + ddH2O; ### p < 0.001 versus NCAPH + ddH2O. Each experiment was repeated at least three times.