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. 2023 Jan 15;56(7):e13400. doi: 10.1111/cpr.13400

FIGURE 6.

FIGURE 6

Non‐SMC condensin I complex subunit H (NCAPH) regulates the cytolysis of CD8 T cells through β‐catenin. (A) FACS analysis of CD8 T cell‐mediated elimination of Caki‐1 cells. &&& p < 0.001 versus Blank; ***p < 0.001 versus vector; ### p < 0.001 versus sh‐NC. (B) Expression of perforin and Granzyme‐B in CD8 T cells after co‐cultured Caki‐1 cells with NCAPH overexpression or depletion was detected by Western bolting. (C–D) The levels of IFN‐γ and TNF‐α secreted by CD8 T cells were measured by ELISA assay. ***p < 0.001 versus vector; ### p < 0.001 versus sh‐NC. (E) PD‐1 mRNA expression in CD8 T cells was measured. &&& p < 0.001 versus Blank; ***p < 0.001 versus vector; ### p < 0.001 versus sh‐NC. (F) Membrane PD‐1 expression in CD8 T cells was measured by flow cytometry. &&& p < 0.001 versus Blank; ***p < 0.001 versus vector; ### p < 0.001 versus sh‐NC. (G) CD8 T cell‐mediated elimination of clear cell renal cell carcinoma cells was measured by flow cytometry after NCAPH depletion or β‐catenin overexpression. ***p < 0.001 versus sh‐NC + vector; ### p < 0.001 versus sh‐NCAPH‐1. Each experiment was repeated at least three times.