Fig. 2. Cellular behaviours of MC3T3-E1 preosteoblasts cultured on 3D printed PLA and GO-PLA films. (a) Cell viability of MC3T3-E1 preosteoblasts on 10, 100, and 1000 μg mL−1 GO-incorporated or pristine PLA films at 24 h, (b) intracellular ROS generation at 24 h, (c) LDH release at 24 h, (d) initial cell adhesion at 6 h, (e) cell migration speed during 48 h, and (f) cell proliferation profiles for 5 DIV. Ph indicates phase contrast. Scale bars of (b) represent 100 μm. The asterisks represent statistical differences (*p < 0.05 and ****p < 0.0001).
