Fig. 3. TCR affinity of CD8 T cells is positively associated with T_RM maintenance and phenotype.

(A) F1.OVA kidney allografts were transplanted into GFP⁺ B6 recipients followed by co-adoptive transfer of 1 × 10⁶ effector OT-I and P14 cells 2 days post-transplantation. Transplant recipients were harvested on days 6 (n = 5) and 56 (n = 4) to quantify CD8 T_RM after gating on extravascular graft CD8⁺ T cells as shown in Fig. S1B.

(B) Percentage (representative d.56 plot, left) and ratio (graph, right) of CD45.1⁺ P14 to CD90.1⁺ OT-I cells on days 6 and 56.

(C) (Balb/c × B6.OVA) F1.OVA kidney allografts were transplanted into GFP⁺ B6 recipients followed by co-adoptive transfer of 1 × 10⁶ CD90.1/90.2⁺ effector OT-I cells and CD45.1⁺ effector OT-3 cells 2 days post-transplantation. Transplant recipients were harvested on days 6 (n = 5) and 56 (n = 4) to quantify CD8 T_RM after gating on extravascular graft CD8⁺ T cells as shown in Fig. S1B.

(D) Percentage (representative d.56 plot, left) and ratio (graph, right) of OT-3 to OT-I cells on days 6 and 56.

(E) Percentage (representative plots, left) of OT-I and OT-3 cells expressing T_RM cell markers and percentage (graph, right) of OT-I and OT-3 cells that were CD69⁺ in the renal allograft on day 56.

P values were determined by (B, D, E) two-tailed paired t test.