Fig. 5. Graft-infiltrating DCs are required for T_RM cell maintenance and graft pathology.

(A) F1.OVA kidney allografts were transplanted into CD11c-DTR:B6 chimeras (B6 mice reconstituted with CD11c-DTR bone marrow) or control B6:B6 chimeras (B6 mice reconstituted B6 bone marrow) followed by adoptive transfer of 1 × 10⁶ effector OT-I cells 2 days post-transplantation. Diphtheria toxin was administered to both groups every other day from days 28–42. Flow analysis was performed after gating on extravascular graft CD8⁺ T cells as shown in Fig. S1B. n = 6 mice per group.

(B) Representative flow plots and absolute number (graph) of intragraft CD11c⁺MHCII⁺ DCs after DT treatment.

(C) Representative flow plots and absolute number (graphs) of T_RM OT-I cells after DT treatment.

(D) Absolute number of recipient-derived polyclonal CD8 and CD4 T_RM cells after DT treatment.

(E) H&E and MT-stained sections of F1.OVA renal allograft tissue (representative images) and quantification of infiltrate and fibrosis (graphs) from bone marrow chimera recipients on day 42 following DT treatment . Whole image scale bar: 500 μm. Inset scale bar: 100 μm.

P values were determined by (B-E) two-tailed unpaired t test.