Figure 6: Mll1 regulates expression of LEF-1 and TCF-1 in T_FH cells.

(A) Volcano plots of gene expression in gCd8⁺ and gMll1⁺ T_FH or T_H1 plotted against adjusted p-value. Genes of interest are indicated. (B) TPM values for Tcf7 and Lef1 in the indicated populations. (C) GSEA of genes down- or up-regulated in Lef-RV⁺ T_H1 compared to genes differentially expressed in gCd8⁺ T_FH and gMll1⁺ T_FH. (D) GSEA analysis of genes down- or up-regulated in Lef1^−/−Tcf7^−/− GC-T_FH compared to genes differentially expressed in gCd8⁺ T_FH and gMll1⁺ T_FH. Selected genes that form the core enrichment signatures are listed. (E-F) shRNA⁺ SMARTA CD4 T cells were transferred into C57BL/6 mice and analyzed 3 days after infection with LCMV-Arm. (E) LEF-1 or (F) TCF-1 expression in CXCR5⁺CD25⁻ SMARTA CD4 T cells. Numbers in the histograms indicate geometric MFI values. Data shown are from one experiment representative of 3 (E-F) independent experiments with 4 mice per group. (B) **p ≤ 0.01, ***p ≤ 0.001 (two-way ANOVA with Sidak’s multiple comparison test), (E-F) *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 (one way ANOVA with Tukey’s multiple comparisons test).