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. Author manuscript; available in PMC: 2024 May 16.
Published in final edited form as: Vaccine. 2023 Apr 24;41(21):3367–3379. doi: 10.1016/j.vaccine.2023.04.042

Figure 2.

Figure 2

Monoclonal (mAb) sexual stage parasite reactivity. Immunoblots of purified gametocyte (gc) or gamete/zygote (gm) Triton X-100 extract without β-mercaptoethanol or gamete/zygote Triton-X-100 extract with (r) and without (nr) β-mercaptoethanol were probed with the indicated mAb or as positive controls, anti-recombinant MBP.Pfs230.C (α230) (A) or MBP.Pfs48/45 (α48/45) serum (B). Previously reported mAb specific for Pfs230 (1B3) (A) or Pfs25 (4B7) (C) are also included as controls. Immunoblots are grouped according to the mAb pattern: A) Pfs230-like, B) Pfs48/45-like, C) Pfs25-like, and D) No reactivity. 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and nitro blue tetrazolium (NBT) were used to visualize the bands.