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. Author manuscript; available in PMC: 2024 May 16.
Published in final edited form as: Vaccine. 2023 Apr 24;41(21):3367–3379. doi: 10.1016/j.vaccine.2023.04.042

Figure 3.

Figure 3

Monoclonal (mAb) gamete/zygote surface reactivity. Purified wild-type strain NF54 (wt) or Pfs230Δ452–3135 (Pfs230Δ) gametes/zygotes were incubated with the indicated mAb, washed, and then stained with Alexa Fluor 488-labeled anti-mouse IgG and membrane potential dye DiIC1 (5). Fluorescence was monitored using flow cytometry and fluorescence microscopy. For each antibody and gamete/zygote population, the DiIC1(5) and Alexa Fluor 488 fluorescence of each cell is plotted and representative fluorescent and bright field images are shown.