Table 3:
Monoclonal antibody characterization.
| Isotype | Gamete Immunoblot | Gamete surface reactivityb | Immuno-precipitation | |||||
|---|---|---|---|---|---|---|---|---|
| TRAa | ID | Full name | Heavy & Light Chain | Non-reduce d | Reduced | WT | 230 minus | |
| 1 | 2C7 | F5 04F5.2C7 | IgG2a, Kappa | 25 | neg | pos | pos | |
| 2 | 1H1 | F3 09B9.1H1 | IgG2a, Kappa | 25 | neg | pos | pos | |
| 3 | 1G9 | F2 14E5.1G9 | IgG1, Kappa | 48 | neg | pos | pos | |
| 4 | 7E5 | F5 06D11.7E5 | IgG2a, Kappa | 230 | neg | pos | neg | |
| 5 | 5E1 | F3 09F4.5E1 | IgG1, Kappa | 230 | neg | pos | neg | |
| 6 | 2F5 | F3 05A6.2F5 | IgG2a, Kappa | 230 | neg | pos | neg | |
| 7 | 2H11 | F3 14A1.2H11 | IgG2b, Kappa | neg | neg | pos | neg | 230 |
| 8 | 11A6 | F6 30C5.11A6 | IgG2a, Kappa | neg | neg | pos | pos | 230 & 47 |
| nonTRA | ||||||||
| 1 | 8F5 | F6 13C10.8F5 | IgG2a, Kappa | 48 | neg | pos | pos | |
| 2 | 2D8 | F4 05H5.2D8 | IgG2a, Kappa | 80–100 | neg | pos | ND | HSP90+ |
| 3 | 4D2 | F3 14D10.4D2 | IgG1, Kappa | 230 | neg | pos | neg | |
| 4 | 5E2 | F3 08F1.5E2 | IgG1, Kappa | 230 | neg | pos | neg | |
| 5 | 7B6 | F3 08D6.7B6 | IgG2a, Kappa | 230 | >230 | pos | neg | |
| 6 | 5B9 | F3 05H1.5B9 | IgG2a, Kappa | neg | neg | pos | neg | |
| 7 | 8G6 | F3 04C6.8G6 | IgG2b, Kappa | neg | neg | pos | neg | |
| 8 | 3F10 | F5 09G9.3F10 | IgG1, Kappa | neg | neg | pos | neg | |
| 9 | 2F8 | F3 06D6.2F8 | IgG1, Kappa | neg | neg | pos | neg | |
| 10 | 1B1 | F4 11D9.1B1 | IgG1, Kappa | neg | neg | pos | neg | |
| 11 | 6C6 | F2 18F12.6C6 | IgG2a, Kappa | neg | neg | pos | pos | |
| Control | ||||||||
| 12 | 1A12 | F2 03G2.1A12 | IgG1, Kappa | neg | neg | neg | ND | |
Characteristics of the 8 transmission-reducing (TRA), 11 non-transmission-reducing (nonTRA), and 1 control monoclonal are listed.
Wild-type (WT) and Pfs230Δ452–3135 gametes/zygotes (230 minus) were used to detect surface reactivity.