Sparse and stereotyped encoding implicates a core glomerulus for ant alarm behavior

. Author manuscript; available in PMC: 2024 Jul 6.

Published in final edited form as: Cell. 2023 Jun 14;186(14):3079–3094.e17. doi: 10.1016/j.cell.2023.05.025

Key Resources Table

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Chicken polyclonal anti-GFP	Abcam	Cat#ab13970; RRID: AB_300798
Rabbit polyclonal anti-RFP	Rockland	Cat#600-401-379; RRID: AB_2209751
Mouse monoclonal anti-SYNORF1	Developmental Systems Hybridoma Bank	DSHB: 3C11; RRID: AB_528479
Mouse monoclonal anti-Orco	Gift from Vanessa Ruta; Butterwick et al.¹³⁶	clone 20F7
Goat anti-Chicken Alexa 488	Invitrogen	Cat#A-11039; RRID: AB_2534096
Donkey anti-Mouse Alexa 647	Invitrogen	Cat#A32787; RRID: AB_2762830
Donkey anti-Rabbit Alexa 594	Invitrogen	Cat# A-21207; RRID: AB_141637
Chemicals, peptides, and recombinant proteins
AlexaFluor 555 phalloidin	Invitrogen	Cat#A34055
DAPI	Invitrogen	Cat#D1306
Paraffin oil	Hampton Research	Cat#HR3-421
100% pentane	Sigma Aldrich	Cat#236705
96% 4-methyl-3-heptanone	Pfaltz and Bauer	Cat#M19160
≥99% 4-methyl-3-heptanol	Sigma-Aldrich	Cat#M48309
99% 6-methyl-5-hepten-2-one	Sigma-Aldrich	Cat#M48805-100ML
95% 4-methyl-3-hexanol	Enamine	Cat# 615-29-2
98% 3-hexanone	Aldrich Chemistry	Cat#103020-10G
98% ethylpyrazine	Sigma-Aldrich	Cat#250384-5G
99% propionic acid	Sigma-Aldrich	Cat#W292419-SAMPLE-K
100% ethanol	Decon Laboratories	Cat#2716
≥99.5% isopropanol	Fisher Chemical	Cat#A416SK-4
RNAClean SPRI XPBeads	Beckman Coulter	Cat#A63987
Critical commercial assays
Endotoxin-free midiprep kit	Machery-Nagel	Cat#740420.10
HiScribe T7 Arca mRNA kit (with tailing)	New England Biolabs	Cat#E2060S
Experimental models: Organisms/strains
O. biroi clonal line A wild type	Kronauer Lab	N/A
O. biroi clonal line B wild type	Kronauer Lab	N/A
O. biroi clonal line B [ie1-DsRed-ObirOrco-QF2-15xQUAS-GCaMP6s]; “GCaMP6s ant”	This paper	N/A
Deposited data
O. biroi reference genome v5.4	McKenzie and Kronauer¹⁰	GCA: 003672135.1
Whole-genome sequence of GCaMP6s ant	This paper	BioProject ID: PRJNA947257
Confocal microscopy data	This paper	Brain Image Library IDs: 7b20a5b168a92088; 626fd578e97289bc; c2031d8218de058b
Calcium imaging data	This paper	DANDI Archive ID: 000467
Oligonucleotides
Primers for plasmid construction; see Table S4	This paper	N/A
Recombinant DNA
plasmid pGL3-IE1	Gift from Zach Adelman; Anderson et al.⁶²	Addgene, ID 52894
plasmid pBAC-ECFP-15xQUAS_TATA-SV40	Gift from Christopher Potter; Riabinina et al.¹²²	Addgene, ID 104875
plasmid pBac-DsRed-ORCO_9kbProm-QF2	Gift from Christopher Potter; Riabinina et al.¹²²	Addgene, ID 104877
plasmid pGP-CMV-GCaMP6s	Gift from Douglas Kim & GENIE Project; Chen et al.⁶⁰	Addgene 40753
plasmid hyPB^apis	Gift from Martin Beye; Otte et al.⁶⁵	N/A
plasmid pBAC-ie1-DsRed-ObirOrco-QF2-15xQUAS-GCaMP6s	This paper	Addgene, ID 200400
Software and algorithms
ImageJ (Fiji) version 2.0.0	Schindelin et al.¹²³	https://fiji.sc/
LABKIT plugin for ImageJ	Arzt et al.¹²⁴	https://github.com/juglab/labkit-ui
Trimmomatic 0.36	Bolger et al.¹²⁵	http://www.usadellab.org/cms/?page=trimmomatic
bwa mem	Li¹²⁶	https://github.com/lh3/bwa
Picard	Broad Institute	http://broadinstitute.github.io/picard/
samtools	Li et al.¹²⁷	https://github.com/samtools/samtools
R	R Core Team¹²⁸	www.R-project.org.
ggplot2	Wickam¹²⁹	https://ggplot2.tidyverse.org
Integrative Genomics Viewer	Robinson et al.¹³⁰	https://software.broadinstitute.org/software/igv/
msa	Bodenhofer et al.¹³¹; Larkin et al.¹³²	https://bioconductor.org/packages/release/bioc/html/msa.html
BLAST	Morgulis et al.¹³³	https://blast.ncbi.nlm.nih.gov/Blast.cgi
karyoploteR	Gel and Serra¹³⁴	http://bioconductor.org/packages/release/bioc/html/karyoploteR.html
Prism	GraphPad Prism	https://www.graphpad.com
Image Stabilizer plugin for ImageJ	Li¹³⁵	http://www.cs.cmu.edu/~kangli/code/Image_Stabilizer.html
napari	napari contributors	Zenodo DOI: 10.5281/zenodo.3555620
Other
Additional scripts	This study	https://github.com/Social-Evolution-and-Behavior/HartKronauer2023