Figure 4.

miR-433-3p downregulation partly reversed the inhibition of silencing lncRNA IGFL2-AS1 on HCT116 cell malignant proliferation. HCT116 cells were transfected with inhibitor-433-3p, with inhibitor-NC as the control. (A) The transfection efficiency of inhibitor-433-3p was detected via qRT-PCR, followed by a collaborative experiment with si-IGFL2-AS1-2. (B) Cell viability was assessed via MTT assay. (C) Cell proliferation was assessed via colony formation assay. Cell experiments were performed 3 times independently. Data were represented as mean ± SD, ***P < .001, **P < .01. Data in figure A were analyzed using unpaired t-test, data in figure B were analyzed using 2-way ANOVA, and data in figure C were analyzed using 1-way ANOVA, followed by Tukey’s multiple comparison test. SD, standard deviation; ANOVA, analysis of variance; lncRNA IGFL2-AS1, long noncoding RNA insulin-like growth factor 2-like family member 2 antisense RNA 1; qRT-PCR, quantitative real-time polymerase chain reaction; MTT, 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide.