Table 1.
Gcm2 KO efficiency in mouse offspring after zygote genome editing using CRISPR-Cas9
| Strain | Embryos transferred | Living fetuses | Genotypes of Gcm2 mutants | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Monoallelic | Biallelic | Mosaic | n.d. | |||||||
| Out-of-frame | In-frame | Both | Out-of-frame | In-frame | Both | |||||
| B6 | 120 | 20 | 0 | 6 | 1 | 7 | 3 | 0 | 3 | 0 |
| BDF1B6 | 100 | 24 | 0 | 9 | 3 | 7 | 0 | 1 | 0 | 4 |
| BDF1 | 60 | 20 | 1 | 5 | 5 | 7 | 0 | 0 | 2 | 0 |
B6, BDF1B6, and BDF1, respectively, mean C57BL/6N, B6D2F1 × C57BL/6N, and B6D2F1. Those that could not be amplified by crude PCR with the mouse primer pairs were described as n.d.