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. 2023 Jul 5;120(28):e2210152120. doi: 10.1073/pnas.2210152120

Fig. 3.

Fig. 3.

LPS inhibits CCh-mediated increase in steady-state current density and IKACh in isolated SAN cells. (A and B) Traces showing mean ± SEM of current density from −100 to +40 mV ramp over 1.5 s and (C) scatter plot with mean ± SEM for current density at −100 mV in the absence (−) and presence (+) of 0.1 µM CCh in the isolated SAN cells from (B) LPS-injected mice (N = 6) vs. (A) controls (N = 12). (D) Scatter plot with mean ± SEM for current density at −100 mV in isolated control SAN cells (N = 8) prefused with 0.1 µM CCh ± 300 nM tertiapin-Q. (E) Representative traces and (F) scatter plot with mean ± SEM representing change in the IKACh outward current density at −35 mV holding potential following 0.1 µM CCh perfusion in SAN cells isolated from LPS-injected mice (N = 5) vs. control mice (N = 6). **P < 0.01, ***P < 0.001, nsP > 0.05; (C) mixed two-way ANOVA followed by simple effect analysis, (D) repeated measure one-way ANOVA followed by within-group effect analysis with Bonferroni adjustment, (F) independent samples t test.