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. 2023 Jul 5;120(28):e2217405120. doi: 10.1073/pnas.2217405120

Fig. 2.

Fig. 2.

The CREBBP/EP300 inhibitor A-485 impairs STB differentiation. A-485 (1 µM) was added to STB differentiation medium and this condition was compared to untreated TSCs and STBs. Cells in TSC medium were analyzed on day 3 and cells in STB medium ± A-485 on day 5 after seeding. (A) Representative phase-contrast and immunofluorescence microscopy images of cells stained for EpCAM (TSC marker, green), hCG (STB marker, magenta) and DAPI (nuclei, white). (Scale bars: 100 µm.) (B) qPCR analysis of TSC marker and STB marker mRNA expression. Bars represent mean log2 fold-change (FC) ± SD relative to TSC medium (n = 4 independent experiments). (C) ELISA for hCGβ secretion into the medium within 24 h. TSC medium was collected between day 2 to 3 and STB medium ± A-485 between day 4 to 5. Bars represent mean fold-change (FC) ± SD relative to TSC medium (n = 3 independent experiments). Color legend corresponds to (B and C). ***P < 0.001.