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. 2023 Jul 3;13(11):3856–3871. doi: 10.7150/thno.82607

Figure 5.

Figure 5

Hepatocyte HSPA12A inhibited HMGB1 secretion from hepatocytes. A-C. Mouse experiments. LI/R was induced in mice (A). Mouse serum was collected to examine HMGB1 protein levels using immunoblotting, and equal volume of serum separated on SDS-PAGE and stained with Ponceau S solution served as protein loading control (B). Also, liver tissues were collected, cytosolic fraction prepared, and immunoblotted for HMGB1 (C). n = 4/group (B) and n = 6/group (C). D-F. Hepatocyte experiments. H/R was induced in primary hepatocytes (D). After then, culture medium was collected, exosome isolated, and immunoblotted for HMGB1 (E). Blotting for HSP70 and Calnexin served as positive and negative markers, respectively (E). Also, primary hepatocytes were immunestained with HMGB1, and DAPI was used to stain nuclei (F). Scale bar = 5 μm. n = 3/group (E) and n = 4/group (F). Data are mean ± SD, ** P < 0.01 by two-way ANOVA followed by Tukey's test.