Table 3.
Antibodies used for immunostaining
| Primary antibody | Dilution | Category NO. | Company | 2nd antibody | Dilution | Category NO. | Company |
|---|---|---|---|---|---|---|---|
| Mouse anti-myosin light chain atrial isoform | 1:200 | 311011AT1 | Synaptic systems | Goat anti-mouse IgG-PE | 1:400 | A31570 | Thermo Fisher |
| Rabbit anti-myosin light chain ventricular isoform | 1:200 | 10906-1-AP | Proteintech | Goat anti-rabbit IgG-FITC | 1:400 | A32790 | Thermo Fisher |
| Mouse anti-cardiac troponin T-PE | 1:20 | 564767 | BD Biosciences | Goat anti-mouse IgG-PE | 1:400 | A31570 | Thermo Fisher |
| Rabbit anti-troponin I3 (cardiac type) | 1:200 | ab-47003 | Abcam | Goat anti-rabbit IgG-FITC | 1:400 | A32790 | Thermo Fisher |
| Rabbit anti-myosin Heavy Chain 6 | 1:200 | 22281-1-AP | Proteintech | Goat anti-rabbit IgG-FITC | 1:400 | A32790 | Thermo Fisher |
| Mouse anti-myosin Heavy Chain 7 | 1:10 | MAB1548 | Millipore | Goat anti-mouse IgG-PE | 1:400 | A31570 | Thermo Fisher |
| Rabbit anti-IRX4 | 1:200 | 104135-T34 | Sino Biological | Donkey anti-rabbit IgG-FITC | 1:400 | 711-095-152 | Jackson ImmunoResearch |