Table 4.
Antibodies used for fluorescence activated cell sorting
Primary antibody | Dilution | Category NO. | Company | 2nd antibody | Dilution | Category NO. | Company |
---|---|---|---|---|---|---|---|
Mouse anti-myosin light chain atrial isoform-APC | 1:600 | 311011AT1 | Synaptic systems | ||||
Rabbit anti-myosin light chain ventricular isoform | 1:600 | 10906-1-AP | Proteintech | Goat anti-rabbit IgG-FITC | 1:800 | A32790 | Thermo Fisher |
Mouse anti-cardiac troponin T-PE | 1:20 | 564767 | BD Biosciences | ||||
Rabbit anti-troponin I3 (cardiac type) | 1:600 | ab-47003 | Abcam | Goat anti-rabbit IgG-FITC | 1:1000 | A32790 | Thermo Fisher |
Rabbit anti-myosin Heavy Chain 6 | 1:600 | 22281-1-AP | Proteintech | Goat anti-rabbit IgG-FITC | 1:1000 | A32790 | Thermo Fisher |
Rabbit anti-myosin Heavy Chain 7 | 1:600 | MAB90961 | Millipore | Goat anti-mouse IgG-FITC | 1:1000 | A32790 | Thermo Fisher |