Table 6.
Antibodies used in Western Blot analysis
| Primary antibody | Dilution | Category NO. | Company | 2nd antibody | Dilution | Company |
|---|---|---|---|---|---|---|
| Rabbit anti-myosin light chain ventricular isoform | 1:500 | 10906-1-AP | Proteintech | Goat anti-rabbit-IgG-HRP | 1:4000 | Cell signaling |
| Rabbit anti-troponin I3 (cardiac type) | 1:1000 | ab-47003 | Abcam | Goat anti-rabbit-IgG-HRP | 1:4000 | Cell signaling |
| Mouse-anti-myosin Heavy Chain 7 | 1:200 | MAB1548 | Millipore | Goat anti-mouse-IgG-HRP | 1:4000 | Cell signaling |
| Mouse anti-SERCA2a | 1:1000 | SC-376235 | Santa Cruz | Goat anti-mouse-IgG-HRP | 1:4000 | Cell signaling |
| Mouse anti-RyR | 1:200 | SC-376507 | Santa Cruz | Goat anti-mouse-IgG-HRP | 1:1000 | Cell signaling |
| Rabbit anti-Kir2.1 | 1:500 | AB5374 | Millipore | Goat anti-rabbit-IgG-HRP | 1:2000 | Cell signaling |
| Mouse Nav1.5 | 1:100 | sc-81631 | Santa Cruz | Goat anti-mouse-IgG-HRP | 1:1000 | Cell signaling |
| Mouse-Cav1.2 | 1:400 | sc-398433 | Santa Cruz | Goat anti-mouse-IgG-HRP | 1:2000 | Cell signaling |
| Mouse anti-MFN1 | 1:500 | sc-166644 | Santa Cruz | Goat anti-mouse-IgG-HRP | 1:4000 | Cell signaling |
| Mouse anti-MFN2 | 1:500 | sc-100560 | Santa Cruz | Goat anti-mouse-IgG-HRP | 1:4000 | Cell signaling |
| Mouse anti-Fis1 | 1:500 | sc-376469 | Santa Cruz | Goat anti-mouse-IgG-HRP | 1:4000 | Cell signaling |
| Rabbit anti-GAPDH | 1:5000 | 2118S | Cell signaling | Goat anti-rabbit-IgG-HRP | 1:5000 | Cell signaling |
| Mouse anti-TET1 | 1:200 | sc-293186 | Santa Cruz | Goat anti-mouse-IgG-HRP | 1:1000 | Cell signaling |
| Rabbit anti-TET2 | 1:200 | GTX124205 | Gene Tex | Goat anti-rabbit-IgG-HRP | 1:2000 | Cell signaling |
| Rabbit anti-IRX4 | 1:500 | 104135-T34 | Sino Biological | Goat anti-rabbit-IgG-HRP | 1:4000 | Cell signaling |