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. 2023 Jul 10;15(1):2233149. doi: 10.1080/19490976.2023.2233149

Figure 2.

Figure 2.

Fucose administration increases Lgr5+ ISCs and promote ISCs proliferation. (a) if analysis of Lgr5-EGFP on ileum sections from control and fucose-treated mice (Scale bar, 100 μm). (b) IHC analysis of Olfm4 on ileum sections from control and fucose-treated mice (Scale bar, 100 μm). (c) Western blot analysis of Lgr5 and Olfm4 in ileum crypts of control and fucose-treated mice. (d) Relative gene expression of Lgr5, Olfm4 and Ascl2 in ileum crypts of control and fucose-treated mice. (e) if analysis of Lgr5-EGFP and Ki67 in ileum crypts of control and fucose-treated mice (Scale bar, 50 μm). (f) EdU staining on ileum sections from control and fucose-treated mice at different timing (Scale bar, 100 μm). (g) Images of organoids derived from control and fucose-treated mice and statistical analysis of surface area and budding number (Scale bar, 50 μm).