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. 2023 Jul 10;15(1):2233149. doi: 10.1080/19490976.2023.2233149

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© 2023 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 6. — Fucose enhanced the property of Akk to promote ISCs stemness via increasing its propionic acid production. (a) Images of organoids that were treated with supernatant of Akk or fucose-treated Akk and EdU staining (Scale bar, 50 μm). Akk+Fuc1: Akk was pretreated with 1 mM fucose, Fuc5: Akk was pretreated with 5 mM fucose. (b) Relative gene expression of Lgr5, Olfm4, Ascl2 Lyz1, Muc2 and ChgA of organoids that were treated with supernatant of Akk or fucose-treated Akk. (c,d) the concentration of SCFAs in the supernatant of Akk and 5 mM fucose-treated Akk.