Figure 7.

Ileal contents from fucose administrated mice promotes organoids development in a Gpr41/Gpr43 dependent manner. (a) Images of organoids that were treated with ileal contents from control and fucose-treated mice and statistical analysis of surface area and budding number (Scale bar, 50 μm). (b) if analysis of Lgr5-EGFP in organoids that were treated with ileal contents from control or fucose-treated mice (Scale bar, 50 μm). (c) Relative gene expression of Lgr5, Olfm4 and Ascl2 of organoids that were treated with ileal contents from control or fucose-treated mice. (d) EdU and Ki67 staining of organoids that were treated with ileal contents from control or fucose-treated mice (Scale bar, 50 μm). (e) if analysis of lysozyme, Muc2 and ChgA on organoids that were treated with ileal contents from control or fucose-treated mice (Scale bar, 50 μm). (f) Relative gene expression of Lyz1, Muc2 and ChgA of organoids that were treated with ileal contents from control or fucose-treated mice. (g) Images of organoids that were treated with ileal contents from control and fucose-treated mice when there were Gpr41/Gpr43 antagonist (Scale bar, 50 μm). (h) EdU staining on organoids that treated with ileal contents from control and fucose-treated mice when there was Wnt-c59 (Scale bar, 50 μm).