Figure 8.

Fucose administration promotes ISCs functions through Wnt signaling pathway. (a) if analysis of β-catenin in ileum crypts of control and fucose-treated mice (Scale bar, 100 μm). (b) Relative gene expression of Wnt3, Axin2 and Ctnnb1 in ileum crypts of control and fucose-treated mice. (c) Western blot analysis of Wnt3, Axin2 and β-catenin in ileum crypts of control and fucose-treated mice. (d) if analysis of β-catenin in organoids that were treated with ileal contents from control or fucose-treated mice. (e) Images of organoids that were treated with ileal contents from control and fucose-treated mice when there were Wnt signaling inhibitor Wnt-c59 (Scale bar, 50 μm). (f,g) Lgr5-EGFP and EdU staining of organoids that were treated with ileal contents from control and fucose-treated mice when there were Wnt signaling inhibitor Wnt-c59 (Scale bar, 50 μm).