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. Author manuscript; available in PMC: 2023 Jul 11.
Published in final edited form as: Analyst. 2020 Oct 26;145(21):6875–6886. doi: 10.1039/d0an01098g

Fig. 1.

Fig. 1

(a) Denaturing PAGE image showing products of the ldh1 iSDA assay in a few selected porous materials. Only Std 17 GF showed the targeted amplification product bands at ~100 and 120 nucleotides (green arrow) comparable to the assay as performed in-tube (conventional polypropylene tube). The inevitable primer dimer side products are indicated with a red arrow. Quartz (QMA), nitrocellulose (NC-FF80), and Fusion 5 totally inhibited amplification, including the primer-dimer side reactions. (b) Gel image showing the effect of leachate from porous materials on iSDA as performed in a tube. Leachate from NC-FF80, Fusion 5, and cellulose significantly inhibited iSDA as seen by very faint product-specific products, whereas the leachate from QMA did not affect the iSDA assay.