Fig. 2.

Effect of combinations of preservatives on ldh1 iSDA with 250 copies of MRSA genomic DNA measured by real-time fluorescence using the target-specific probe. The control sample was without any preservatives. Curves are mean of 3 replicates, and error bars are standard deviation. The preservatives did not have any negative effect on iSDA. The lift-off times of the reactions were nearly identical (~7 min), and the peak fluorescence levels were similar for all the formulations (T = 10% trehalose, TP = 10% trehalose + 1% PEG, TD70 = 10% trehalose + 2.5% dextran (~70 kDa), TD500 = 10% trehalose + 2.5% dextran (500 kDa) and NTC was a no-template control).