Fig. 6.
LOD of ldh1 iSDA with reagents stored dry in Std 17 GF at 22 °C and 45 °C for 360 h in different formulations of the preservatives. (a) Normalized intensity of the LF strip test line for samples stored at 22 °C. Samples with trehalose and dextran (TD 70 & TD 500) in the iSDA mix showed excellent stability down to 10 genomic copies. Samples with either trehalose only (T) or trehalose and PEG (TP) in the iSDA mix had diminishing signal intensity with decreasing genomic copy numbers. (b) Normalized intensity of the LF strip test line for samples stored at 45 °C. Excellent stability down to 10 copies was achieved for samples with trehalose and dextran (TD 70 & TD 500), whereas samples with trehalose (T) only or trehalose and PEG (TP) showed poor stability.