Fig. 4.

(a) Pixel-by-pixel heatmap of Fc/AA versus DD/AA of CHO-K1 cells expressing TSMod. 269 cells from 6 samples with exposure times of 100 or 200 ms and ND filter of OD 1 (around 48 millions pixels). The pixel values are fitted with a 2D Gaussian: $f(x,y)=A\exp (-{((x-x_0)/w_x)}^2-{((y-y_0)/w_y)}^2)$. $R^2=\mathrm{0,967}$. The black cross represents the center of the fitted Gaussian, and the dashed ellipse is the 95% confidence bound of the Gaussian. The center of the Gaussian is given by $x_0=0.3366\pm 1.2e^{-4}$ and $y_0=0.2005\pm 8.0e^{-5}$. The red and white lines represent Eqs. (2) and (3), respectively. (b) Projections of the 2D histogram along $x$ and $y$ axes.