Fig. 6.

(a) VinTS consists of TSMod inserted between the vinculin head and tail. (b) Vinculin tail-less sensor (VinTL) is a control construct only linked to the vinculin head. (c), (d), (g), and (h) Fluorescence images in the acceptor channel of cells expressing VinTS and VinTL. (e), (f), (i), and (j) The FRET efficiencies averaged over the segmented FAs of cells expressing VinTS and VinTL. Images (c-f) are taken on the Paris-Saclay setup with an exposure time of 1900 ms. Images (g-j) are cropped images of $1100\times 1100\text{\hspace{0.17em}\hspace{0.17em}pixels}$ from the total field of view of $2048\times 2048\text{\hspace{0.17em}\hspace{0.17em}pixels}$, taken on the Rutgers setup with an exposure time of 1000 ms.