Fig 3. TAL1-short promotes transcription from TSS1-3.

(A) HEK293T cells were cotransfected with TAL1 promoters: promoters 1–3, 4, 5, and exon 4 as a negative control. The second plasmid was an empty vector, TAL1-short or TAL1-long. After 30 h, luciferase activity was calculated relative to renilla. Horizontal broken lines indicate transfection with the empty luciferase. The mean was calculated from 3 independent biological experiments, each performed with 3 technical replicates (**P < 0.01, Student t test). (B) HEK293T cells were cotransfected with TAL1 5′ UTRs: TSS 1, 2, 4, and 5 and empty vector as negative control. After 30 h, luciferase activity was calculated relative to renilla. The mean was calculated from 3 independent biological experiments, each performed with 3 technical replicates (*P < 0.05, Student t test) are shown. Underyling data can be found in S1 Data.