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. 2023 Jun 28;21(6):e3002175. doi: 10.1371/journal.pbio.3002175

Fig 6. TAL1-short is a stronger differentiation agent.

Fig 6

(A-D) K562 cells were infected with inducible shRNA against the 3′ UTR of TAL1. In addition, the cells were infected with MIGR1 plasmid with GFP at the C-terminal followed by an IRES element. Infection was with empty vector, TAL1-short, or TAL1-long. Following induction with tetracycline for 72 h, cells were treated with 20 μM hemin to promote erythroid differentiation for the indicated time points. RNA was extracted and analyzed by real-time PCR for total mRNA amount of α-, β-, and γ-globin and SLCA4 relative to CycloA and hTBP reference genes without hemin (A) and with treatment (inducing differentiation) for 48 h (B). Cells were seeded and counted every day following labeling by trypan blue. Live cells are plotted in (C) and trypan blue-labeled cells are plotted in (D). Plots represents the mean of 3 independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, Student t test). Underyling data can be found in S1 Data.