Figure 1.

Workflow for preparing native membrane-protein libraries reflecting the P. aeruginosa membrane proteome. Pseudomonas aeruginosa membrane fractions were harvested from cell lysates by ultracentrifugation. Membrane proteins were solubilized from the membranes with the polymer DIBMA, spontaneously forming nanodiscs containing membrane proteins embedded in a lipid bilayer. The nanodiscs were separated and fractionated by size exclusion chromatography. Proteins from the collected fractions were precipitated and subsequently analyzed by mass spectrometry. The relative abundance of the identified proteins was plotted against the elution volume in chromatographic separation.