Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 22;14:1204224. doi: 10.3389/fimmu.2023.1204224

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2023 Graham, Gazinska, Zhang, Khiabany, Sinha, Alaguthurai, Flores-Borja, Vicencio, Beuron, Roxanis, Matkowski, Liam-Or, Tutt, Ng, Al-Jamal, Zhou and Irshad

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Proteome profiles of secreted factors from nonstimulated and BC cell line EV-stimulated healthy volunteer PBMCs. (A) Representative human XL cytokine array dot blots are shown. (B) A heatmap of the analysis of dot blot intensity across all experimental conditions is shown. (C) Bar chart showing significant changes in analytes detected in PBMC culture supernatants stimulated with a cell line-derived EVs: (i) MDA-MB-231, (ii) MCF-7, (iii) HCC1954, and (iv) BT474-derived EVs compared to unstimulated PBMC. Upregulated analytes are illustrated in red and those downregulated are shown in blue. (D) The Venn diagram is used to identify overlapping and nonoverlapping up- or downregulated cytokines in the analysis of nonstimulated compared to MDA-231, MCF-7, HCC1954, and BT474 EV stimulated. (E) REACTOME pathway analysis of the commonly altered cytokines in EV-stimulated immune cells compared to nonstimulated immune cells. (F) Pathway analysis of specific analytes up- or downregulated in the supernatant of PBMC co-cultures with MDA-231, MCF-7, HCC1954, and BT474 EVs.