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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: Methods Mol Biol. 2023;2644:349–359. doi: 10.1007/978-1-0716-3052-5_22

Figure 3.

Figure 3.

Representative image of migrated cells stained with crystal violet. J774A.1 macrophage/monocyte migration using 100 ng/mL recombinant mouse C5a. Migrated cells are stained with 0.2% crystal violet solution. Images of the transwell membrane are taken with a 20x objective using the ECHO Revolve 3 microscope. Migrated J774A.1 cells are stained purple. Scale bar = 100 μm.