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. 2023 Jun 15;55(7):1221–1231. doi: 10.1038/s41588-023-01419-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, Plant and ear photos of Mo17. b, Whole-genome coverage of ONT reads across the basal Mo17 assembly. Ultralong ONT reads longer than 10 kb were used for coverage analysis. The LCRs with reads depth lower than 100 and high-coverage regions (HCRs) with reads depth higher than 250 were marked by black shades. c,d, Schematic representation showing that a gap (c) and LCR (d) on the basal Mo17 assembly were closed or corrected by the contigs of PacBio Hifiasm assembly, in which the validity was confirmed by uniform ONT reads coverage and tiling ONT reads. e, Validation of the final assembly of the terminal 1 Mb regions for chromosome 4. Red pentagrams indicated the ONT reads used to correct the telomere length for corresponding chromosomal ends, in which the telomeric repeats harbored by them were longer than other reads mapped to corresponding ends. f, Schematic representation showing the manual closing for a TAG repeat array-related gap on chromosome 2 by ONT reads. g, Validation of the assembly of the TE-rich region in the 45S rDNA array by ONT reads. The red arrows represent the transcriptional directions of 45S rDNAs.